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geldoc1000 image analysis station  (Bio-Rad)


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    Structured Review

    Bio-Rad geldoc1000 image analysis station
    Geldoc1000 Image Analysis Station, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/geldoc1000+image+analysis+station/geldoc+1000/pm30738800-24-67-71
    Average 90 stars, based on 1 article reviews
    geldoc1000 image analysis station - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: p39R861-4, A Type 2 A/C2 Plasmid Carrying a Segment from the A/C1 Plasmid RA1.
    Article Snippet: .. Amplicons were resolved by electrophoresis on 1% (w/v) agarose gels with molecular weight standards, stained with ethidium bromide, and visualized using a GelDoc1000 image analysis station (BioRad). .. Total plasmid DNA was extracted and sequenced on an Ion Torrent platform (Life Technologies) as previously described.11 The sequencing reads (62-fold coverage) were assembled de novo into contigs using Geneious version 6.1.6 (Biomatters).

    Article Title: Analysis of pCERC7, a small antibiotic resistance plasmid from a commensal ST131 Escherichia coli, defines a diverse group of plasmids that include various segments adjacent to a multimer resolution site and encode the same NikA relaxase accessory protein enabling mobilisation.
    Article Snippet: .. Electrophoresis (120 V) was performed for 2 h before the gel was stained with ethidium bromide (5 μg/mL) and imaged using a GelDoc1000 image analysis station (BioRad). ..

    Article Title: IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated Acinetobacter baumannii GC1 and GC2 Clones
    Article Snippet: .. PCR amplicons were resolved by electrophoresis on 1% (wt/vol) agarose gels with molecular weight standards, stained Volume 1 Issue 3 e00117-16 msphere.asm.org 8 on July 29, 2016 by guest http://m sphere.asm .org/ with ethidium bromide, and visualized using a GelDoc1000 image analysis station (Bio-Rad). .. Separated PCR products were purified for sequencing by using the QIAquick gel extraction kit (Qiagen Inc., Valencia, CA, USA), following the manufacturer’s protocols.

    Article Title: An improved plasmid size standard, 39R861.
    Article Snippet: The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s.. However, their sizes had only been estimated.. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861–3 is reported, and the sizes of p39R861–4, pSa and NTP168 were determined to be 155.8, 40.1 and 6.8 kb, respectively.

    Molecular Weight:

    Article Title: p39R861-4, A Type 2 A/C2 Plasmid Carrying a Segment from the A/C1 Plasmid RA1.
    Article Snippet: .. Amplicons were resolved by electrophoresis on 1% (w/v) agarose gels with molecular weight standards, stained with ethidium bromide, and visualized using a GelDoc1000 image analysis station (BioRad). .. Total plasmid DNA was extracted and sequenced on an Ion Torrent platform (Life Technologies) as previously described.11 The sequencing reads (62-fold coverage) were assembled de novo into contigs using Geneious version 6.1.6 (Biomatters).

    Article Title: IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated Acinetobacter baumannii GC1 and GC2 Clones
    Article Snippet: .. PCR amplicons were resolved by electrophoresis on 1% (wt/vol) agarose gels with molecular weight standards, stained Volume 1 Issue 3 e00117-16 msphere.asm.org 8 on July 29, 2016 by guest http://m sphere.asm .org/ with ethidium bromide, and visualized using a GelDoc1000 image analysis station (Bio-Rad). .. Separated PCR products were purified for sequencing by using the QIAquick gel extraction kit (Qiagen Inc., Valencia, CA, USA), following the manufacturer’s protocols.

    Staining:

    Article Title: p39R861-4, A Type 2 A/C2 Plasmid Carrying a Segment from the A/C1 Plasmid RA1.
    Article Snippet: .. Amplicons were resolved by electrophoresis on 1% (w/v) agarose gels with molecular weight standards, stained with ethidium bromide, and visualized using a GelDoc1000 image analysis station (BioRad). .. Total plasmid DNA was extracted and sequenced on an Ion Torrent platform (Life Technologies) as previously described.11 The sequencing reads (62-fold coverage) were assembled de novo into contigs using Geneious version 6.1.6 (Biomatters).

    Article Title: RSF1010-like plasmids in Australian Salmonella enterica serovar Typhimurium and origin of their sul2-strA-strB antibiotic resistance gene cluster.
    Article Snippet: Salmonella enterica serovar Typhimurium phage type 9 isolates resistant to streptomycin and sulfonamide have been recovered from both bovine and human sources in Australia.. This study aimed to identify the resistance genes and their location.. Polymerase chain reaction was used to screen for resistance genes and sul2 (sulphonamide resistance) and strA and strB (streptomycin resistance) were detected.

    Article Title: Analysis of pCERC7, a small antibiotic resistance plasmid from a commensal ST131 Escherichia coli, defines a diverse group of plasmids that include various segments adjacent to a multimer resolution site and encode the same NikA relaxase accessory protein enabling mobilisation.
    Article Snippet: .. Electrophoresis (120 V) was performed for 2 h before the gel was stained with ethidium bromide (5 μg/mL) and imaged using a GelDoc1000 image analysis station (BioRad). ..

    Article Title: IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated Acinetobacter baumannii GC1 and GC2 Clones
    Article Snippet: .. PCR amplicons were resolved by electrophoresis on 1% (wt/vol) agarose gels with molecular weight standards, stained Volume 1 Issue 3 e00117-16 msphere.asm.org 8 on July 29, 2016 by guest http://m sphere.asm .org/ with ethidium bromide, and visualized using a GelDoc1000 image analysis station (Bio-Rad). .. Separated PCR products were purified for sequencing by using the QIAquick gel extraction kit (Qiagen Inc., Valencia, CA, USA), following the manufacturer’s protocols.

    Article Title: pCERC1, a small, globally disseminated plasmid carrying the dfrA14 cassette in the strA gene of the sul2-strA-strB gene cluster.
    Article Snippet: Commensal Escherichia coli from healthy adult humans were screened for antibiotic resistance genes.. Two unrelated strains contained the sul2 sulphonamide resistance gene and strAB streptomyicn resistance genes with the dfrA14 trimethoprim resistance gene cassette in the strA gene and conferred resistance to trimethoprim and sulphamethoxazole.. A 6.8 kb plasmid, pCERC1, that contains these resistance genes was recovered and sequenced.

    Article Title: Plasmids in antibiotic susceptible and antibiotic resistant commensal Escherichia coli from healthy Australian adults.
    Article Snippet: 32 A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy 33 Australian adults were screened using PCR-based replicon typing.. Each isolate represented a 34 distinct strain found in a particular faecal sample.. 51 isolates were resistant to one or more of 12 35 antibiotics tested.

    Agarose Gel Electrophoresis:

    Article Title: RSF1010-like plasmids in Australian Salmonella enterica serovar Typhimurium and origin of their sul2-strA-strB antibiotic resistance gene cluster.
    Article Snippet: Salmonella enterica serovar Typhimurium phage type 9 isolates resistant to streptomycin and sulfonamide have been recovered from both bovine and human sources in Australia.. This study aimed to identify the resistance genes and their location.. Polymerase chain reaction was used to screen for resistance genes and sul2 (sulphonamide resistance) and strA and strB (streptomycin resistance) were detected.

    Article Title: pCERC1, a small, globally disseminated plasmid carrying the dfrA14 cassette in the strA gene of the sul2-strA-strB gene cluster.
    Article Snippet: Commensal Escherichia coli from healthy adult humans were screened for antibiotic resistance genes.. Two unrelated strains contained the sul2 sulphonamide resistance gene and strAB streptomyicn resistance genes with the dfrA14 trimethoprim resistance gene cassette in the strA gene and conferred resistance to trimethoprim and sulphamethoxazole.. A 6.8 kb plasmid, pCERC1, that contains these resistance genes was recovered and sequenced.

    Article Title: Plasmids in antibiotic susceptible and antibiotic resistant commensal Escherichia coli from healthy Australian adults.
    Article Snippet: 32 A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy 33 Australian adults were screened using PCR-based replicon typing.. Each isolate represented a 34 distinct strain found in a particular faecal sample.. 51 isolates were resistant to one or more of 12 35 antibiotics tested.

    Polymerase Chain Reaction:

    Article Title: IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated Acinetobacter baumannii GC1 and GC2 Clones
    Article Snippet: .. PCR amplicons were resolved by electrophoresis on 1% (wt/vol) agarose gels with molecular weight standards, stained Volume 1 Issue 3 e00117-16 msphere.asm.org 8 on July 29, 2016 by guest http://m sphere.asm .org/ with ethidium bromide, and visualized using a GelDoc1000 image analysis station (Bio-Rad). .. Separated PCR products were purified for sequencing by using the QIAquick gel extraction kit (Qiagen Inc., Valencia, CA, USA), following the manufacturer’s protocols.

    Plasmid Preparation:

    Article Title: An improved plasmid size standard, 39R861.
    Article Snippet: The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s.. However, their sizes had only been estimated.. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861–3 is reported, and the sizes of p39R861–4, pSa and NTP168 were determined to be 155.8, 40.1 and 6.8 kb, respectively.

    Alkaline Lysis:

    Article Title: An improved plasmid size standard, 39R861.
    Article Snippet: The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s.. However, their sizes had only been estimated.. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861–3 is reported, and the sizes of p39R861–4, pSa and NTP168 were determined to be 155.8, 40.1 and 6.8 kb, respectively.

    Pulsed-Field Gel:

    Article Title: An improved plasmid size standard, 39R861.
    Article Snippet: The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s.. However, their sizes had only been estimated.. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861–3 is reported, and the sizes of p39R861–4, pSa and NTP168 were determined to be 155.8, 40.1 and 6.8 kb, respectively.

    Nucleic Acid Electrophoresis:

    Article Title: pCERC1, a small, globally disseminated plasmid carrying the dfrA14 cassette in the strA gene of the sul2-strA-strB gene cluster.
    Article Snippet: Commensal Escherichia coli from healthy adult humans were screened for antibiotic resistance genes.. Two unrelated strains contained the sul2 sulphonamide resistance gene and strAB streptomyicn resistance genes with the dfrA14 trimethoprim resistance gene cassette in the strA gene and conferred resistance to trimethoprim and sulphamethoxazole.. A 6.8 kb plasmid, pCERC1, that contains these resistance genes was recovered and sequenced.

    Article Title: Plasmids in antibiotic susceptible and antibiotic resistant commensal Escherichia coli from healthy Australian adults.
    Article Snippet: 32 A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy 33 Australian adults were screened using PCR-based replicon typing.. Each isolate represented a 34 distinct strain found in a particular faecal sample.. 51 isolates were resistant to one or more of 12 35 antibiotics tested.



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    Bio-Rad geldoc1000 image analysis station
    Geldoc1000 Image Analysis Station, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/geldoc1000+image+analysis+station/geldoc+1000/pm30738800-24-67-71
    Average 90 stars, based on 1 article reviews
    geldoc1000 image analysis station - by Bioz Stars, 2026-09
    90/100 stars
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